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To quantify actinic arrest in brilliance and autofluorescence assays, bristles assays with thirteen readouts in absolute were run. One appraisal abstinent firefly luciferase (Fluc) inhibition beneath cell-free altitude and four assays, consisting of two corpuscle types (HepG2 and HEK-293) and two ability altitude (cell-based and cell-free application ability boilerplate only), abstinent autofluorescence. Anniversary autofluorescence appraisal had three beaming access readouts (blue, blooming and red with filters of emission/excitation according to 485/535 nm, 405/460 nm, and 540/590 nm, respectively). The Tox21 actinic library of 8305 altered structures were buried in quantitative high-throughput format; see Methods for details.
The Tox21 actinic library is composed of 9,667 substances and 8,305 altered structures excluding mixtures and ions. The Tox21 library was congenital from a federal cross-agency accomplishment accoutrement a ample actinic amplitude as discussed in16,42,43, and includes assorted actinic groups e.g. pesticides, antimicrobials, baptize contaminants, automated chemicals, aerial assembly aggregate chemicals, endocrine disruptors, FDA aliment additives, fragrances, plasticizers and drugs. The library contains abounding automated chemicals prioritized based on abeyant for animal exposure, which are not advised to be bioactive and accordingly accept a college abeyant to baffle with the assays because they accept not been filtered out by alleviative allure attempt acclimated in developing baby atom libraries.
To appraise the actinic advantage of the Tox21 library, chemicals were projected on a Principal basic Appraisal (PCA) authentic application the Broadcast Structure-Searchable Toxicity (DSSTox) database accoutrement the bigger curated ecology actinic library with added that 800,000 chemicals. Figure 1 shows that the Tox21 chemicals are able-bodied broadcast on the PCA authentic from the DSSTOX application 1D and 2D atomic descriptors, see Methods, and accommodate ample advantage of the structural landscape
Principal basic appraisal of 596,526 chemicals (QSAR accessible structures) from the DSSTOX database, authentic application 1D and 2D descriptors from RDKit library, see methods, accoutrement 33.3% of structural variability. (A) represents the bump on the map of the DSSTOX chemicals and (B) the 8,305 altered structures from the Tox21 actinic library, assuming analogously ample structural coverage.
Figure 2A shows the acknowledgment ambit for Ataluren (PTC-124; anatomy apparent in admit console 1) the advertence absolute ascendancy actinic for the luciferase inhibition assay. PTC-124 has an 3,5-diaryl oxadiazole arch circuitous with a m-carboxylate which binds Fluc in the ATP alive site. The alternation modifies the α-phosphate of ATP through a SN2 displacement acknowledgment and inhibits Fluc9,44. The acknowledgment ambit shows accelerated arresting abridgement with an estimated IC50 (based on leash measurements) beneath 20 nM. The acknowledgment ambit chic was −1.1, which corresponds to a complete inhibition response, see Methods and Supporting Advice Table S1 18.
Examples of acknowledgment curves for absolute ascendancy chemicals: (A) Ataluren – PTC124 (1. 775304-57-4) in luciferase inhibition appraisal (B) Tiramterene (2. 396-01-0) in autofluorescence assay, dejected spectrum, (C) Fluorescin (3. 2321-07-5) in autofluorescence assay, blooming spectrum and (D) Rose bengal sodium (4. 632-69-9) in autofluorescence assay, red spectrum.
For autofluorescence assays, advertence chemicals were the fluorophores Tiramterene (insert console 2), fluorescein (3) and rose bengal (4) for blue, blooming and red channels, respectively. Acknowledgment curves are presented in Fig. 2B–D for anniversary of the four appraisal altitude (HEK-293 cell-based, HEK-293 cell-free, HepG2 cell-based, and HepG2 cell-free). All advertence chemicals acknowledgment curves showed a cogent concentration-dependent fluorescence increase. The arresting was stronger for the blooming access with AC50s <4 µM (curve chic 1.1, complete activation response). For the dejected and red channels, the AC50s were beneath 40 µM and 12 µM, respectively, with acknowledgment ambit classes according to 2.1, agnate to an abridged acknowledgment (due to abridgement of added asymptote) but with >80% efficacy.
The Tox21 actinic library of 8305 altered structures was buried application autofluorescence and luciferase inhibition assays to anon admeasurement technology interference. Table 1 summarizes the cardinal of alive chemicals by assay. A actinic was authentic as alive if it anesthetized all of the filters, i.e. AC50/IC50 cutoff, ambit class, and ability for luciferase and autofluorescence, and cytotoxicity cutoffs for autofluorescence only. Supporting advice Table S2 contains the dataset afore and afterwards filtering. For luciferase inhibition assays 6.6% of the chemicals were begin to be active, with an boilerplate IC50 of 28.3 /− 19.1 µM. For autofluorescence assays, the dejected access had the accomplished cardinal of alive chemicals with amid 2.5 to 2.7% of the library depending on corpuscle types and ability conditions. In the blooming access amid 0.5 to 0.9% of the chemicals were begin to be alive and amid 0.4 to 0.5% in the red channel. The boilerplate AC50 ranged from 16.5 to 22.3 µM depending on the access and altitude for autofluorescence and 28.3 for luciferase.
To characterize actinic arrest action by actuality blazon and use case, the Tox21 actinic library was classified application the customer articles database45, the Toxic Substances Ascendancy Act actinic account (TSCA), and accustomed drugs lists accessible in the EPA actinic dashboard (https://comptox.epa.gov/dashboard). Based on these resources, 4950 of the 8305 altered chemicals included in the Tox21 actinic library could be classified into 80 classes. The best busy classes of alive chemicals are represented in Fig. 3. Interferent chemicals are begin apart of technology or action in assorted actinic classes including chemicals with ablaze assimilation backdrop i.e. UV cushion (enriched for luciferase inhibition), beard dye (enriched for autofluorescence), photo architect and colorants. Drugs and TSCA classes were additionally begin to be accomplished in arrest chemicals. Specifically, luciferase interferents were begin mostly in preservative, pesticide and antioxidant actinic classes. Chemicals causing autofluorescence in the dejected access were begin in bark conditioner, antioxidant and aroma classes, while red and blooming access interferents were accomplished for drugs, TSCA and beard dye classes.
Classification of arrest chemicals for the 19 best busy actinic classes (from 80 classes). (A) Actinic counts for the 4950 chemicals classified from Tox21 chemicals library. (B) Top actinic classes with arrest action for luciferase inhibition and blue, blooming and red access autofluorescence.
The administration of action of called alive chemicals for anniversary appraisal is apparent in Fig. 4 as a body plot. For luciferase inhibition assays, the IC50 administration (Fig. 4A) shows a body aiguille about 1.5 log(µM) and specific actinic inhibitory concentrations broadcast below. The distributions of alive chemicals in autofluorescence assays (Fig. 4B–D) arise to be artless by any aggregate of corpuscle blazon and ability conditions. Interestingly, the action body plots for all three-color wavelengths authenticate bimodal authority distributions, with two peaks about 1 and 1.6 log(µM), and the majority of AC50 ethics falling beneath 2 log(µM). The bimodal authority administration was begin for the best busy ambit classes administration as appearance in Supporting Advice Fig. S1. For compounds with abridged curves, e.g. curves that did not ability a acknowledgment plateau, the absolute AC50 could be college than the accomplished analysis concentration, but to abstain accepting an extrapolated ambit of AC50s that are not reliable, our ambit applicable algorithm tries to bind the AC50 to beneath the accomplished analysis concentration, appropriately basic the 2nd aiguille in the distribution.
Distributions of (A) log(IC50) (µM) for luciferase inhibition assay; and (B–D) log(AC50) (µM) for autofluorescence assays application HepG2 and HEK-293 ability beef and cell-free (medium only) conditions. AC50 (µM) were abstinent for the autofluorescence assays in the (B) blue, (C) blooming and (D) red spectrum. The boilerplate of anniversary administration is represented application a vertical line.
From the Tox21 library, 8,065 altered chemicals were extracted afterward anatomy curation, and were amassed application a SOM access and a set of 165 non-correlated and advisory 1D-2D descriptors, see methods. Chemicals were amassed in a SOM set with 225 clusters, acceptance acceptable allegory of the chemicals with alone two clusters abandoned and one singleton, and two clusters with beneath than ten chemicals. On boilerplate anniversary array is composed of 36 /− 14 chemicals administration agnate structural backdrop (Supplementary Advice Fig. S2).
The SOM was black application the allotment of alive chemicals begin in anniversary cluster, Fig. 5A. Alive chemicals were broadcast beyond 154 clusters with 2 /− 3 alive chemicals per cluster, and an boilerplate allotment of alive chemicals according to 11 /− 11% with a best according to 47%. Three clusters that are accomplished for chemicals alive in the luciferase inhibition appraisal are accent and archetype structures shown. Array 163 had 33% (n = 6) alive chemicals, and included three-ring structures circuitous with at atomic one phosphate accumulation and some ketone and booze groups, see archetype structures 5, 6 and 7. Phosphate accumulation substructures can bind the ATP bounden armpit of Fluc and block its activity9. Array 144 had 43% (n = 4) alive chemicals, and included at atomic bristles rings structures not ramified, see archetype structures 8, 9 and 10. Array 129, in abutting adjacency on the SOM map, had 44% (n = 10) alive chemicals and included at atomic bristles affiliated rings circuitous with alcohol, ketone or chlorine for example, structures not shown. Array 99, had 47% (5) alive chemicals, area structural scaffolds included biphenyl groups (example structures 11, 12) or a diphenyldiazene (13) circuitous primary amines groups (11, 12 and 13).
Structure-based SOM on the 8,065 structure-curated Tox21 chemicals, including 225 clusters in total, (A) black application percent alive chemicals in luciferase inhibition assays (6.6% of tox21 chemicals), and (B) black application percent alive chemicals in autofluorescence assays (4.7% of tox21 chemicals). Left-hand blush scales represent the allotment of alive actinic by cluster. Archetype actinic structures for clusters accomplished for action are displayed; array 163: 5. 860-22-0, 6. 7, 6. 129-17-9, 7. 25956-17-6; array 144: 8. 2531-84-2, 9. 193-39-5, 10. 50-32-8 and array 99: 11. 7411-49-6,12. 531-85-1, 13. 136-40-3; array 203: 14. 84-36-6, 15. 22260-51-1, 16. 131-01-1; array 147: 17. 130-17-6, 18. 93-00-5, 19.547-91-1; array 117: 20. 654055-01-3, 21. 518-47-8, 22. 20004-62-0.
The aforementioned structure-based SOM run on the absolute Tox21 library was afresh black according to the allotment of alive chemicals by array on all of the autofluorescence assays alloyed together, Fig. 5B. The set of alive auto beaming chemicals was composed of 379 chemicals broadcast beyond 134 clusters with 3 /− 2 alive chemicals per array and an boilerplate allotment of alive chemicals of 9.2 /− 8.8%. Three clusters that are accomplished for alive chemicals in the autofluorescence assays are accent and archetype structures shown.
Cluster 203 had 56% (n = 10) alive chemicals and included ample chemicals with added than 6 rings (example structures 14, 15 and 16). Chemicals in array 147 (44% alive chemicals, n = 8) independent two conjugated rings circuitous with one phosphate and, for example, primary amine (17, 18) or iodine (19). Finally, array 117 had 30% (n = 9) alive chemicals and contains anatomy with not added than three conjugated rings circuitous with booze groups. (example structures 20, 21, and 22).
The SOM was afresh black by allotment of alive actinic by blush access agnate to blue, green, and red wavelengths, Fig. 6A–C, respectively. The 379 alive chemicals in autofluorescence assays were disconnected into 315 actives for the blue, 95 for the blooming and 40 for the red channel, see Table 1.
Structure based SOM on the 8,065 Tox21chemicals, black by the allotment of alive chemicals by array for autofluorescence assays in blush channels: (A) blue, (B) blooming and (C) red. Archetype actinic structures for clusters accomplished for action are displayed; array 203: 23. 25614-03-3, 24. 131.01-1, 25. 100286-90-6; array 147: 26. 93-00-5, 27. 130-17-6, 28. 82956-11-4; array 129: 29. 1210-12-4, 30. 2693-46-1, 31. 3331-46-2; array 209: 32. 25316-40-9, 33. 50935-04-1, 34. 75443-99-1; array 173: 35. 17692-51-2, 36. 60-79-7, 37. 159701-44-7; array 117: 38. 6151-25-3, 39. 517-28-2, 40. 1470-79-7; array 209: 41. 25316-40-9, 42. 72496-41-4, 43. 56124-62-0; array 219: 44. 16423-68-0, 45. 115-39-9; array 143: 46. 514-73-8, 47. 905-97-5.
From the three clusters discussed above, none arise to be specific for one blush and alone array 203 was begin to additionally be accomplished in the dejected access (56% alive chemicals, n = 10). Added archetype structures are apparent (23, 24 and 25) which accept a ample arch including added than six rings circuitous mostly with oxygen groups, e.g. booze or ketone.
Structural clusters with specific accessory for the dejected access accommodate 147 and 129 with 44% and 40% (8 and 10 chemicals) alive in anniversary cluster, respectively. Array 147 is composed of chemicals with sulfonic acids (example structures 26, 27) or a guanidium basement (28) affiliated to an ambrosial arena such as naphthalene or benzene. Array 129 includes chemicals with a baby arch constituted by one accumulation of several affiliated ambrosial rings, e.g. anthracene (three ambrosial cycles, 29), fluoranthene (30), or pyrene (four cycles, 31).
For the blooming channel, array 209 is the array with the accomplished allotment of alive chemicals with 27% actives apery eight chemicals. Alive chemicals are composed of at atomic one unsaturated arena circuitous with oxygen groups such as alcohol, ketone or carboxylic acerbic (example structures 32, 33 and 34), and do not accommodate heteroatoms added than oxygen and carbon. Array 173 is additionally displayed in Fig. 6, with alone 8% actives on the blooming access but apery bristles chemicals, all of which accommodate a diazatetracyclohexadeca pentaene and terminal alternation composed by nitrogen and oxygen accumulation (example anatomy 35, 36 and 37). Array 117 is additionally accomplished for autofluorescence on the blooming amicableness with seven actives apery 23% of the abounding cluster, accoutrement ambrosial rings circuitous with alone booze or ketone accumulation (example structures 38, 39, and 40). These chemicals are structural analogues of the fluorescein atom with a aerial amount of arch similarity.
In the red access array 209 was afresh accomplished with 27% actives, agnate to eight chemicals. All of the alive chemicals in this array were autofluorescent in both the blooming and red access and included at atomic one unsaturated arena circuitous with oxygen groups such as alcohol, ketone or carboxylic acerbic (additional archetype structures 41, 42 and 43).
Cluster 194 included two chemicals apery 18% (n = 4) of the array actives with anatomy composed by three aromatics circuitous with iodine (44), bromine (45) and additionally chlorine, not shown. Finally, array 143 additionally included three alive chemicals apery 17% of the cluster. Structurally, chemicals accommodate on both extremities a benzothiazole affiliated with an unsaturated alternation (example structures 46 and 47), and no oxygens.
For anniversary blush access (blue, green, and red), autofluorescence action was abstinent application two corpuscle types (HepG2 and HEK-293) and two ability conditions, cell-free (culture boilerplate only) and cell-based. As apparent with the SOM, some chemicals approved action beyond blush channels. Figure 7 quantifies the overlap amid autofluorescent chemicals by blush access (Fig. 7A) and aural anniversary amicableness by corpuscle line/culture action (Fig. 7B–D).
Venn diagrams of alive chemicals for autofluorescence assays, (A) comparing altered blush channels, (B) because alone the dejected access and comparing corpuscle lines/culture conditions, (C) because alone the blooming access and comparing corpuscle lines/culture conditions, and (D) because alone the red access and comparing corpuscle lines/culture conditions. For specific blush channels corpuscle ability altitude are represented as corpuscle based (hepg2_cell and hek293_cell) and corpuscle free, ability boilerplate alone (hepg2_free and hek293_free).
Only 3.5% of the 404 autofluorescent chemicals were alive beyond all corpuscle ability altitude and blush wavelengths, Fig. 7A. The 14 chemicals are apparent in Supporting Advice Table S3. Structurally these chemicals included usually a ample arch with a circuitous ambrosial arena adjustment composed of added than 3 rings. Figure 7B–D defines the overlap of alive chemicals amid corpuscle ability conditions, aural a blush channel. A actinic that is autofluorescent in every aggregate of corpuscle ability action can be advised acerb interferent with the blush access with a aerial confidence; however, these cases represented alone a allocation of the alive chemicals: 40% of actives in the blue, 36% in the blooming access and 53% for the red channel. The overlap amid autofluorescence assays and the luciferase inhibition appraisal is apparent in Supplemental Fig. S3.
The access of four altered corpuscle ability altitude (HepG2 and HEK-293 corpuscle curve with cell-based and cell-free conditions) on actinic action in the autofluorescence assays was advised beyond the three-color channels. To analyze clusters of chemicals accurately alive for one corpuscle ability or action for altered blush channels, hierarchical absorption was performed area alive chemicals administration agnate physico-chemical or topological backdrop were amassed together. The absorption is presented application a annular dendrogram, for anniversary blush access and corpuscle ability action (Fig. 8A–C) and black by potency. Chemicals accurately alive for assertive beginning altitude are accent and structures shown. Hierarchical absorption of autofluorescent chemicals beyond all twelve combinations of corpuscle ability altitude and blush channels is apparent via annular dendrogram in Supporting Advice Fig. S4, and for all alive chemicals in the luciferase inhibition appraisal in Fig. S5.
Hierarchical absorption of alive chemicals in autofluorescence assays for (A) blue, (B) blooming and (C) red channel. Hierarchical absorption is accomplished application a Euclidean ambit computed from a set of non-redundant atomic descriptors and application Ward segregation, see methods. Authority in agreement of AC50 for anniversary actinic is represented application a blush calibration from red to blooming and for the four corpuscle ability conditions, a. Hepg2 corpuscle based, b. Hepg2 corpuscle free, c. HEK-293 corpuscle based and d. HEK-293 corpuscle free. Some actinic structures in alive actinic clusters specific to one corpuscle culture/condition are represented; 48. 15721-78-5, 49. 508-85-2, 50. 144-68-3, 51. 6217-54-5, 52. 127-40-2, 53. 6983-79-5, 54. 149-91-7, 55. 118-75-2, 56. 118-75-2, 57. 77-92-9, 58. 218151-78-1, 59. 85100-78-3, 60. 111991-09-4, 61. 962-58-3, 62. 9-51-7, 63. 6258-06-6, 64. 88-63-1, 65. 2835-99-6, 66. 83-26-1, 67. 481-85-6, 68. 58-27-5, 69. 6264-66-0, 70. 212141-51-0, 71. 132-32-1, 72. 159701-44-7, 73. 17479-19-5, 74. 25447-66-9, 75. 6153-64-6, 76. 5794-24-1, 77. 106-50-30, 78. 7199-29-3, 79. 2243-61-0, 80. 2445-76-3, 81. 54646-38-7, 82. 115-39-9.
Among chemicals alive aural wavelengths, assertive actinic appearance were begin to be associated with action for one corpuscle ability condition. Chemicals 62, 63, 64, 65 and 79 were begin alive accurately on the HepG2 culture. These chemicals accept baby structural scaffolds, beneath than 20 atoms with not added than three conjugated rings such as benzoindole or naphthalene. Similarly, chemicals 49, 57, 66, 67, 68, 69 and 77 are alive alone on HEK-293 and allotment one benzene circuitous with primary amine or booze groups. These structural scaffolds may drive arrest with the ability altitude rather than the blush channels.
Specific to the dejected channel, chemicals 50, 51, 52 and 53, absolute unsaturated chains circuitous with several methyl groups, collaborate accurately with the HEK-293 cell-based and cell-free condition. Chemicals 58 and 59 composed of an imidazole arena affiliated with an aliphatic chain, were alive alone in the HEK-293 cell-free action and chemicals 60 and 61 composed of a pyrimidine and a phosphate accumulation are additionally begin accurately alive on HEK-293 cell-free condition.
Finally, some of the chemicals represented in Fig. 8 (structures 48, 54, 55, 56, 72, 73, 74, 75, 79, 80 and 81) included actinic substructures important for fluorescence, e.g. aerial cardinal of ambrosial rings and are abutting structural analogues of chemicals already discussed in the SOM results.
As apparent application unsupervised statistical approaches, chemicals that actively arrest luciferase allotment agnate structural actinic backdrop and, separately, those that baffle with autofluorescence assays accept accepted features, in some cases specific to arrest patterns apprenticed by appraisal platform, blush channel, corpuscle or condition. Based on structural and physico-chemical properties, quantitative structure–activity relationships (QSAR) models were developed to adumbrate the likelihood of chemical-assay interference. Multiple apparatus acquirements approaches (see Methods) were acclimated to adumbrate luciferase inhibition, autofluorescence in any blush access beyond corpuscle ability conditions, autofluorescence alone by blush access behindhand of conditions, and autofluorescence by blush access and corpuscle ability altitude uniquely. All archetypal architecture steps, including undersampling from the over-represented set of inactives in the Tox21 dataset, training, cross-validation, and testing, were again ten times to ensure that all chemicals were congenital in the process, and the beggarly and accepted aberration of anniversary achievement archetype beyond all ten iterations were appear for anniversary apparatus acquirements approach.
First, QSAR models were congenital to adumbrate luciferase inhibition. Afterwards abstracts processing, anniversary abounding set of chemicals for archetypal architecture was composed of 1,724 chemicals (30% actives, 70% inactives; Table 2). The RF archetypal accomplished the best performance, with a cross-validation MCC according to 0.557 /− 0.016 and analysis set MCC of 0.571 /− 0.045, acknowledging the adherence of the archetypal and the bound overfitting in animosity of the aerial achievement on the training set (MCC of 0.996 /− 0.002). MCC is an all-embracing anticipation metric (where 0 is accidental and 1 is perfect) anecdotic alternation amid predicted and abstinent ethics that considers the alterity arrangement amid alive and abeyant chemicals in the abstracts set (see Methods).
LDA, NN and SVM with a beeline atom (SVM-linear) apparent abutting achievement to the RF QSAR models, with cross-validation MCC according to 0.51 /− 0.015, 0.453 /− 0.031 and 0.486 /− 0.0014, respectively, but the CART and SVM nonlinear models (SVM-radial and SVM-sigmoid) were weaker (cross-validation MCC of 0.442 /− 0.026, 0.109 /− 0.014 and 0.136 /− 0.025, respectively). In the analysis set SVM-sigmoid performed bigger than CART archetypal with MCC according to 0.128 /− 0.068 and −0.467 /− 0.045, respectively.
For autofluorescence assays, QSAR models were aboriginal developed to adumbrate action after appropriate by blush access or corpuscle ability conditions; achievement metrics are appear in Table 3. Alone chemicals that were alive beyond all altitude for anniversary blush (independently) were acclimated for the alive training set. For anniversary clay iteration, the abounding set included 507 chemicals (30% actives, 70% inactives). Agnate to QSAR models developed for luciferase inhibition, RF gave the best achievement with a cantankerous validation MCC according to 0.564 /− 0.026 and analysis set MCC of 0.568 /− 0.094, and CART, SVMs, NN and LDA QSAR models had lower performance.
Specific QSAR models were afresh developed for anniversary blush channel. A actinic was advised alive if it was alive on at atomic one corpuscle ability action in a accurate blush channel. Achievement metrics are presented in Table 4. The clay dataset for the dejected access consisted of 1,045 chemicals, for the blooming access 339 chemicals, and for the red access 148 chemicals. As with the antecedent QSAR models, RF performed bigger in anniversary case followed by SVM-linear, NN and SVM nonlinear/CART. The accomplished assuming color-specific archetypal was on the red channel, with MCCs according to 0.691 /− 0.043 and 0.672 /− 0.092 on the cantankerous validation and analysis set, respectively, followed by the blooming access with cantankerous validation and analysis set MCCs of 0.642 /− 0.025 and 0.651 /− 0. 085 respectively. Performances are lower on the dejected access with MCCs of 0.431 /− 0.028 and 0.438 /− 0.075 for the cantankerous validation and analysis set. However, in the red channel, the accepted aberration associated with the MCC beggarly is college than added blush channels.
Supplemental Tables S4–S7 appearance all archetypal performances for any aggregate of corpuscle ability action and blush channel. On the dejected access QSAR models had weaker achievement admiration autofluorescence beneath cell-based altitude with a cantankerous validation MCC of about 0.44, due to the set of 111 chemicals after circumstantial dejected access action on added corpuscle ability altitude (Fig. 7). For the added culture-specific QSAR models, the performances are abutting to the blush specific QSAR models’ achievement presented in Table 4, due to a aerial amount of bendability amid the red and blooming actives aural anniversary access beyond conditions.
The capricious accent array of the RF QSAR archetypal descriptors are presented in Fig. 9 for luciferase and Fig. 10 for autofluorescence assays and are abbreviated in Supporting Advice Table S8. For all models, the best important descriptors accommodate at atomic one affection anecdotic the polarizability of the actinic (CombDipolPolariz or bcutp) and one of the afterward physicochemical backdrop (UI, logP_pred, BP_pred, MP_pred or BioDegHL_pred). Specifically, for luciferase inhibition the best important descriptors characterize the arrangement amid unsaturated/saturated bonds (Sp3Sp2HybRatio), followed by the E-state of a methyl affiliated to an ambrosial (S12). On average, alive chemicals accept a lower arrangement amid unsaturated/saturated bonds (0.21 for actives and 0.48 for inactives) and accept an activity accompaniment added afflicted by methyl accumulation affiliated to an ambrosial than abeyant (S12) (11.15 for actives and 5.74 for inactives, Supporting Advice Table S9). For autofluorescence QSARs, unsaturated basis (UI) descriptors, which characterize the arrangement amid unsaturated and saturated bonds in a chemical, were begin in about every archetypal as an important descriptor. The unsaturated basis is college for alive chemicals (~3.5) than abeyant (~2.6) due to the actuality that alive actinic are mostly accomplished in ambrosial groups. Accountability descriptors (bcutp), which characterize accumulation by atom blazon and bond, are additionally present in every archetypal and accountability descriptors are college for alive chemicals than inactive. The red access archetypal includes three descriptors anecdotic the accuse (QNmin, Qmin and QOmin) and alive chemicals are beneath answerable that inactives. The QSAR models congenital alone on the dejected and blooming blush channels aggregate six of the top 10 important descriptors, and the dejected access and luciferase inhibition models aggregate four important descriptors.
Variable accent plots for the top 10 descriptors from the accidental backwoods QSAR models admiration luciferase inhibition. Ten ethics for anniversary descriptor are reported, agnate to anniversary of the ten models developed with altered abstracts set segregations. Descriptors are authentic in Supporting Advice Table S8.
Variable accent plots for the top 10 descriptors from the accidental backwoods QSAR models admiration autofluorescence in: (A) any blush channel, (B) the dejected channel, (C) the blooming access and (D) the red channel. The accent of anniversary descriptor is normalized, and ten ethics for anniversary descriptor are appear agnate to anniversary of the ten models developed with altered abstracts set segregations. Descriptors are authentic in Supporting Advice Table S8.
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