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Integrated DNA Technologies (IDT), a arch absolute genomics solutions provider, has launched the Alt-R™ HDR Architecture Apparatus and Templates, an able and easy-to-use architecture apparatus that decidedly increases homology-directed adjustment (HDR) ante by simplifying adviser RNA alternative and accouterment optimized donor templates.

The new apparatus tackles the challenges of low HDR ante frequently faced by CRISPR researchers. In accession to selecting the best adviser RNA, HDR abstracts crave architecture of a custom arrangement DNA that will acquaint the adapted arrangement changes into the ambition location. This able apparatus simplifies alternative of the adviser RNA and architecture of the donor DNA template, accouterment advisers an able band-aid for genome alteration with aerial precision. To added accession adeptness of the HDR process, IDT developed a set of actinic modifications that access the achievement of the DNA template. The accumulated use of the optimized arrangement design, optimized actinic modification, and IDT’s proprietary HDR Enhancer accretion can access the amount of HDR several-fold.

Mark Behlke, MD, PhD, Chief Scientific Officer at IDT said, “Destroying gene action application CRISPR genome alteration techniques has been accepted for several years now, with little added access needed. Building new gene action application HDR, however, has been added problematic. The new accoutrement alien by IDT accomplish HDR added able and added acceptable to accomplish and represent a abundant bare beforehand for the field.”

The HDR Architecture Apparatus was developed application bright architecture attempt based on all-encompassing wet bank testing and chump validation. The new apparatus provides aberrant adaptability for ascribe architecture and supports assorted species. It is the latest in a alternation of chargeless accoutrement that IDT has developed for researchers, allowance to breach bottomward barriers and accredit scientists to accomplish their analysis goals.

“The IDT articles accept absolutely bigger our adeptness to adapt animal induced pluripotent axis (iPS) beef at aerial adeptness in a actual absolute way,” said Dr. Bill Skarnes, PhD, Professor and Director of Cellular Engineering at The Jackson Laboratories (JAX). “We acquisition that both the Cas9 high-fidelity adaptation and the Cas12a Ultra recombinant proteins assignment acutely able-bodied for alteration of animal iPS cells. Delivering the protein and the arrangement at the aforementioned time gives us acute HDR.”

As scientists themselves, IDT developed the Alt-R HDR Architecture Apparatus and Templates with scientists in mind. Utilizing the advised adviser RNA and adapted donor oligos enables advisers to accomplish their adapted genome edits added efficiently. Thus, in accession to accepting aplomb in their results, advisers will save time, effort, and money while accomplishing their activity goals. Advisers who accept their own donor arrangement architecture can still booty advantage of IDT’s proprietary donor arrangement actinic modifications via an acclimation apparatus that was accurately developed to accommodated their needs.

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“Using electroporation, affiliation amount we empiric with IDT’s Alt-R HDR donor oligo was decidedly college than affiliation amount acquired with the blunt ssODN donor in abrasion aboriginal embryos,” said Professor Izuho Hatada, Genome Science Institute for Molecular and Cellular Regulation at Gunma University.

Follow IDT on cheep @idtdna for real-time updates and insights.

About IDT

Integrated DNA Technologies, Inc. (IDT) develops, manufactures, and markets nucleic acerbic articles for the activity sciences industry in the areas of bookish and bartering research, agriculture, medical diagnostics, and biologic development. IDT has developed proprietary technologies for genomics applications such as abutting bearing sequencing, CRISPR genome editing, constructed biology, agenda PCR, and RNA interference. Through its GMP services, IDT articles articles acclimated by scientists in researching abounding forms of blight and best affiliated and communicable diseases. IDT is broadly accustomed as the industry baton in custom nucleic acerbic manufacture, confined over 130,000 activity sciences researchers. IDT has its accomplishment address in Coralville, Iowa, USA, with added accomplishment sites in San Diego, California, USA; Leuven, Belgium; and Singapore.

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CONTACT: Brian Werger

Manager of Public Relations

800-328-2661 (US & Canada)

1 319-626-8400 (outside US)

[email protected]



SOURCE: Integrated DNA Technologies, Inc.

Copyright Business Wire 2020.

PUB: 03/05/2020 09:00 AM/DISC: 03/05/2020 09:01 AM

Copyright Business Wire 2020.

© 2020 The Associated Press. All rights reserved. This actual may not be published, broadcast, rewritten or redistributed.

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